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The Shanghai Yetuo fixed wavelength spectrophotometer is an optical analysis instrument specifically designed to measure the absorption of light at specific wavelengths. Compared to adjustable wavelength or full wavelength spectrophotometers, its wavelength is usually pre-set and mainly suitable for quantitative analysis of specific substances, such as nucleic acids, proteins, chemical reagents, etc.
This instrument is based on the principle of selective absorption of specific wavelengths of light by substances, and calculates the concentration or content of the sample by measuring the degree of absorption of incident light by the sample. Due to its simple structure, convenient operation, and high stability, fixed wavelength spectrophotometers have a wide range of applications in fields such as biomedicine, environmental monitoring, food testing, and industrial quality control.
Basic components of Shanghai Yetuo fixed wavelength spectrophotometer
Light source: Provides stable monochromatic light. Common light sources include halogen lamps (visible light) and deuterium lamps (ultraviolet light).
Monochromator: Typically, a filter or fixed prism is used to select light of a specific wavelength.
Sample Room: Used for placing colorimetric dishes, usually standard 1 cm optical path colorimetric dishes.
Detector: Photomultiplier tube (PMT) or photodiode, used to receive transmitted light and convert it into an electrical signal.
Signal processing system: converts optical signals into absorbance or transmittance and displays them on the instrument screen.
Characteristics of Shanghai Yetuo Fixed Wavelength Spectrophotometer
Single or multiple fixed wavelengths: suitable for specific analytical tasks, such as 280 nm for protein detection, 260 nm for nucleic acid detection, and 600 nm for fine culture OD value determination.
Easy to operate: No need to adjust the wavelength, measurement can be done immediately after turning on, and the experimental process is efficient and convenient.
Measurement stability: The optical system has no movable parts, reducing wavelength shift and improving long-term stability.
Lower cost: Compared to full wavelength spectrophotometers, fixed wavelength models are more affordable and suitable for daily analytical applications.
The main applications of fixed wavelength spectrophotometers
Biomedical: Protein, DNA, RNA concentration determination, enzyme activity detection.
Environmental monitoring: analysis of water pollutants, heavy metals, and chemical reagent concentrations.
Food testing: testing for food additives, pigments, and vitamin content.
Chemical analysis: determination of compound concentration, study of chemical reaction kinetics.
summarize
The Shanghai Yetuo fixed wavelength spectrophotometer is an efficient and stable analytical instrument suitable for rapid quantitative detection of specific substances. Its simple structure and high cost-effectiveness make it one of the indispensable basic equipment in laboratories.
